ATG7 anticorps (C-Term)

N° du produit ABIN6990575

L’anticorps Lapin Polyclonal anti-ATG7 a été validé pour WB, ELISA, IF et ICC. Il convient pour détecter ATG7 dans des échantillons de Humain et Souris.

Aperçu rapide pour ATG7 anticorps (C-Term) (ABIN6990575)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-ATG7 anticorps

Épitope: AA 590-640, C-Term

Specificité: At least three isoforms of APG7 are known to exist, this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Purification: APG7 Antibody is affinity chromatography purified via peptide column.

Immunogène: APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.

Isotype: IgG

Information d'application

Indications d'application: APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: APG7 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: APG7

Sujet: APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Poids moléculaire: Predicted: 69, 74, 77 kDa

Observed: 77 kDa

ID gène: 10533

NCBI Accession: NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy